bambusicola, including hypocrellin A, hypocrellin B, hypocrellin C and hypocrellin D (Fang et al. Hypocrellins are the main secondary metabolites of S. Shiraia bambusicola is an important and valuable macrofungus in the medical and food industries. When am圓65- 1 and vgb were coexpressed, relative expression levels of zftf and pks reached their highest levels at 72 h under liquid fermentation, hypocrellin production reached the highest level 75.85 mg/gds which was 2.99-fold compared with wild type strain within 11 days under SSF, and residual starch of solid substrates was decreased from 35.47 to 14.57%. Furthermore, expression of VHb decreased SSF period. Results demonstrated that expressions and coexpression of AMY365-1 and VHb not only increased the productions of biomass, amylase, hypocrellin, but also up-regulated relative expression levels of four central carbon metabolism genes ( pdc, ald, acs, acc) and seven hypocrellin biosynthesis genes ( fad, mono, zftf, omef, msf, pks, mco). bambusicola were investigated and three overexpression transformants were constructed. To solve these problems, overexpressions of am圓65- 1 and vgb in S. However, during SSF process of this filamentous fungus, use ratio of corn substrate and dissolved oxygen supply are two main limiting factors, which influence production cost, yield and product quality. Shiraia bambusicola is an important and valuable macrofungus and hypocrellins are its main secondary metabolites which have been widely applied in many medical fields.

Hemoglobin gene serial cloner